Mouse anti Yeast (Saccharomyces cerevisiae) TUP1
| Code | Size | Price |
|---|
| EXA-X1538M | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: 10
Regulatory Status: RUO
Target Species: Yeast
Application: Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blotting (5-10 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Yeast Tup 1p-Ssn6p repressor complex represents a novel paradigm for transcriptional repression and for the role of chromatin in repression. TUP 1 and SSN6 are involved in repression of several diverse families of genes in yeast, including cell type-specific genes regulated by the alphga2 and a1/alpha2 repressors. Ssn6-Tupl interacts with class I histone deacetylases required for repression, and rfecrfuitment of yeast Tuplp-Ssn6p repressor is associated with localized decreases in histone acetylation.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Recombinant protein derived from the amino acids 1-200 of yeastt TUP1 protein
Positive Control:
Mav108 cells expressing recombinant TUP1 protein
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
General transcriptional corepressor TUP1, Glucose repression regulatory protein TUP1, Flocculation suppressor protein, Repressor AER2, TUP1, AAR1, AER2, AMM1, CYC9, FLK1, SFL2, UMR7, YCR084C, YCR84
UniProt:
P16649 (Yeast-Saccharomyces cerevisiae)
References
1. Bone, J.R. and Roth, S.Y. 'Recruitment of the yeast Tup1p-Ssn6p repressor is associated with localized decreases in histone acetylation.' J. Biol. Chem 2001, 276, 1808-1813
2. Watson, A.D., et al. 'Ssn6-Tup1 interacts with class I histone deacetylase required for repression.' Genes and Development 2000, 14, 2737-2744
3. Yang, X., et al. 'Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: Coupling protein O-GlcNAcylation to transcriptional repressor.' Cell 2002, 110, 69-80
4. DeSilva, H., et al. 'Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor.' Genetics 1998, 148, 657-667
2. Watson, A.D., et al. 'Ssn6-Tup1 interacts with class I histone deacetylase required for repression.' Genes and Development 2000, 14, 2737-2744
3. Yang, X., et al. 'Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: Coupling protein O-GlcNAcylation to transcriptional repressor.' Cell 2002, 110, 69-80
4. DeSilva, H., et al. 'Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor.' Genetics 1998, 148, 657-667