Sheep anti BrdU (bromodeoxyuridine)

Applications: Western blotting: 25 -100 ?g/ml; Immunoprecipitation: 25-100 ?g/ml; Immunohistochemistry: 25-100 ?g/ml The antibody has been tested using immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 ug/ml, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100?g/ml.
Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody.

Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Cell Cycle

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.

Unconjugated

Products are stable for one year from purchase when stored properly

Ammonium Sulfate Precipitation

Sheep were immunized with bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.

sheep anti BrdU, Bromodeoxyuridine

N/A