Mouse anti Human Keratin (4568101318)

Detect keratins 4,5,6,8,10,13 & 18 by Western blot. Optimal concentration should be evaluated by serial dilutions. Other Applications: Frozen Tissue Staining, Paraffin Section, Immunohistochemistry.

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.Reacting with a variety of keratins (4,5,6,8,10,13 and 18) which reacts with a variety of normal reactive and neoplasmic epithelia. Reacting with simple epithelium and both basal and superbasal layers of comfying and non comfying squamous epithelium this antibody is also useful in staining cultured epithelial cell lines. It is useful in differentiating epithelial tumors from non-epithelial tumors.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Cellular Structures

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from human A431 carcinoma cells and mouse myeloma cells.

Unconjugated

Products are stable for one year from purchase when stored properly

Purified by saturated ammonium sulphate precipitation

Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from human A431 carcinoma cells and mouse myeloma cells.

P13647