Mouse anti Human Keratin 13 & 10

Product Code: EXA-X1252M

Code	Size	Price

EXA-X1252M

100 ug

£316.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG2a

Antibody Clonality: Monoclonal

Antibody Clone: DE-K13

Regulatory Status: RUO

Target Species: Human

Applications:

Immunohistochemistry- Frozen Section (IHC-F)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Shipping:

Ship at ambient temperature freeze upon arrival

Storage:

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:

Antibody detects cytokeration protein numbers 13 and 10 (molecular weights 53 and 56.6 kDa, respectively, according to Moll's classification) by Western blot. Does not react with other cytokeratins or other proteins. Optimal concentration should be evaluated by serial dilutions.

Background:

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.This antibody reacts with 53 kDa and 56.6 kDa cytokeratin (CK) proteins by Western blot. These proteins correspond to CK numbers 13 and 10, respectively, according to Moll's classification1. Using formalin-fixed, paraffin-embedded tissue sections, this antibody detects only the CK13. With frozen sections, this antibody serves as a differentation-related marker for all stratified epithelia and stains all suprabasal cell in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Caution:

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Concentration:

See vial for concentration

Field of Interest:

Cellular Structures

Formulation:

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis:

Western Blotting

Immunogen:

Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from HeLa cells and mouse myeloma cells.

Product Form:

Unconjugated

Product Stability:

Products are stable for one year from purchase when stored properly

Purification Method:

Protein A/G Chromatography

UniProt:

P13646

References

1. Ivanyi, D., et al. Modulation of mammary carcinoma cell phenotype and keratin expression patterns by retinoic acid. Cancer Lett, 1993, 73, 191-205.

2. Ivanyi, D., et al. Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas. Am. J. Vet. Res. 1993, 54, 1095-1102.

3. Ivanyi, D., et al. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. American Journal of Veterinary Research, 1992, 53, 304-314.

4. Ivanyi, D., et al. Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis. Cancer Research, 1990, 50, 5143-5152.

5. Ivanyi, D., et al. New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva. J of Pathol, 1989, 159, 7-12.

6. Moll, R., et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982, 31, 11-24