Mouse anti Human Keratin 5 &14

Detects Keratin 5 and 14 by Western blot. Detects a 58 kDa band, corresponding to Keratin 5, and a 50 kDa band, corresponding to Keratin 14. Antibody can be used for Western blotting and immunohistochemistry on frozen tissues section (1-5 ?g/ml). Does not work on paraffin embedded tissue sections. Optimal concentration should be evaluated by serial dilutions.

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Cellular Structures

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Hybridoma produced by the fusion of splenocytes from mice immunized with cytokeratin enriched extract of feline tongue epithelium and mouse myeloma cells.

Unconjugated

Products are stable for one year from purchase when stored properly

Protein A/G Chromatography

Hybridoma produced by the fusion of splenocytes from mice immunized with cytokeratin enriched extract of feline tongue epithelium and mouse myeloma cells.

P02533