Mouse anti Human Keratin 7
| Code | Size | Price |
|---|
| EXA-X1258M | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: C35
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Frozen Section (IHC-F)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Detects keratin 7 by Western blot. Detects a 54 kDa band corresponding to keratin 7. Optimal concentration should be evaluated by serial dilutions.
Background:
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 7 is a basic cytokeratin which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of the ovary, lung and breast but not colon or gastrointestinal tract.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Cellular Structures
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from mice immunized with purified human keratin 7 isolated from HeLa cytoskeleton and mouse myeloma cells.
Product:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Source:
Hybridoma produced by the fusion of splenocytes from mice immunized with purified human keratin 7 isolated from HeLa cytoskeleton and mouse myeloma cells.
UniProt:
P08729
References
1. Neoplasma 1990, 37, 333-342
2. J. Tumor Marker Oncol. 1990, 5, 219
3. Int. J. Cancer 1988, Suppl. 3, 50-55
2. J. Tumor Marker Oncol. 1990, 5, 219
3. Int. J. Cancer 1988, Suppl. 3, 50-55