Mouse anti Human Calpain I II (small subunit) conjugated with agarose

Application: Use at 0.5-1 ug/ml for ELISA and western blot, 1-2ug/ml for immunoprecipitation.

The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and ?-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, ?-calpain is active at micromolar (?M) concentrations of Ca ++. In addition to the ubiquitously expressed m- and ?calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Cellular Structures

Provided as a concentration of 1 mg/ml in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol, 3mM sodium azide.

Western Blotting

Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.

Agarose

Antibodies are stable for one year from purchase if stored frozen

Protein A/G Chromatography

Mouse anti calpain I, II small subunit, p30, agarose conjugate

P17655