Mouse anti Human Calpain I II (small subunit)
Code | Size | Price |
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EXA-X1012 | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 28F3
Regulatory Status: RUO
Target Species:
- Bovine (Cattle)
- Human
Applications:
- Enzyme Immunoassay (EIA)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
Room Temperature
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Application: Use at 0.5-1 ug/ml for ELISA and western blot, 1-2ug/ml for immunoprecipitation.
Background:
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and ?-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, ?-calpain is active at micromolar (?M) concentrations of Ca ++. In addition to the ubiquitously expressed m- and ?calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Cellular Structures
Formulation:
Provided as a concentration of 1 mg/ml in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol, 3mM sodium azide.
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.
Product:
Provided as a concentration of 1 mg/ml in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol, 3mM sodium azide.
Product Form:
Unconjugated
Product Stability:
Antibodies are stable for one year from purchase if stored frozen
Purification Method:
Protein A/G Chromatography
Source:
Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.
Synonyms:
Mouse anti calpain I, II small subunit, p30
UniProt:
P17655
References
1. Adamec, E., et al. 'Calpain I activation in rat hippocampal neurons in culture is NMDA receptor selective and not essential for excitotoxic cell death.' Brain Res. Mol. Brain Res. 1998, 54, 35-48
2. Guttmann, R.P. et al. 'Oxidation inhibits substrate proteolysis by calpain I but not autolysis.' J. Biol. Chem. 1997, 272, 2005-2012
2. Guttmann, R.P. et al. 'Oxidation inhibits substrate proteolysis by calpain I but not autolysis.' J. Biol. Chem. 1997, 272, 2005-2012