Mouse anti Human Tissue Inhibitor of Matrix Metalloproteinase 1 (TIMP-1)

Antibody can be used for Western blotting (1-2 ?g/ml) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 ?g/ml). Optimal concentration should be evaluated by serial dilutions.

TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases-1, -2, -3 and -4) complex with metalloproteinases such as collagenases, gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 was found to be identical to EPA (erythroidpotentiation activity). Parathyroid hormone has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart and low levels of TIMP-4 are expressed in liver, brain, lung, thymus and spleen.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Cellular Structures

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human TIMP-1 protein and mouse myeloma Ag8563 cells. Sequence common in monkey

Colon and gastric tissues. Stronger expression observed in tumor versus normal protein.

Unconjugated

Products are stable for one year from purchase when stored properly

Protein A/G Chromatography

TIMP-1; CLG1; EPA; HCI

P01033 (Human)Q95KL9 (Macaca)