Sheep anti Human Calreticulin

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Nordic MuBio
Product Code: EXA-Z100P
Product Group: Primary Antibodies
Supplier: Nordic MuBio
CodeSizePrice
EXA-Z100P250 ug£316.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Sheep
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:
Antibody can be used for Western blotting (1:1000-1:5000 dilution) and immunofluorescence (1:50 dilution). Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.
Background:
Calreticulin, a Ca2+ chaperone, is an abundant highly conserved calcium binding protein found in the lumen of the endoplasmic reticulum of eukaryotic cells. This lumen protein can be used to measure the intactness of membrane preparations. It is used as a marker for subcellular fractionation of cells.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Cellular Structures
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Recombinant protein derived from the carboxy terminus of the calreticulin protein fused to KLH.
Positive Control:
MDCK cells; MCF-7 cells. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Ammonium Sulfate Precipitation
Source:
Sheep were immunized with a recombinant protein derived from the carboxy terminus of the calreticulin protein fused to KLH.
UniProt:
Q6IAT4 (Human) P14211 (Mouse) P18418 (Rat) P28490 (Dog)

References

1. Kageyama, S., et al. 'Urinary calreticulin in the diagnosis of bladder urothelial carcinoma.' Int. J. Urol. 2009, 16, 481-486

2. Silerova, M., et al. 'Characterization, molecular cloning and localization of calreticulin in Eisenia fetida earthworms.' Gene 2007, 397, 169-177

3. Souto-Padron, T., et al. 'Immunocytochemical localisation of calreticulin in Trypanosoma cruzi.' Histochem. Cell Biol. 2004, 122, 563-569