Collagen Substrate FITC Conjugate
| Code | Size | Price |
|---|
| EXA-X1098 | 10 mg | £480.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Bovine (Cattle)
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Used for the assay of mammalian collagenase activity. To heat denature collagen for preparing a substrate for gelatinase, transfer collagen solution to a brown glass bottle and heat to 80OC.
Background:
FITC-labeled bovine type I Collagen Substrate is an excellent substrate for examining collagenase activity. This labelled substrate is highly purified and telo-peptide free and is supplied lyophilized. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. FITC-labeled collagen can also be used as a substrate for cell culture. Collagen degradation products in the culture supernatants can be directly determined by measured the flourescence at 520 nm (Emission)/490 nm (Excitation).
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Cellular Structures
Formulation:
Provided as a 1 mg/ml solution in 0.01M acetic acid.
Functional Analysis:
Activity Assay
Immunogen:
Bovine skin
Label:
FITC
Product:
FITC-labeled bovine type I Collagen provided as a 1 mg/ml solution in 0.01M acetic acid.
Product Form:
FITC
Product Stability:
Stable in acidic buffer when stored at -20OC, however slowly degrades when stored in under neutral conditions.
Purity:
>95%
Source:
Bovine skin
We certify that:
a) The collagen fluorescein is of animal origin only.
b) The collagen fluorescein was derived only from animals which were slaughtered in the United States
in USDAinspected abattoirs where the animals were subjected to ante- and post mortem inspection
and the animals were fit for use in laboratory studies.
c) The United States if free from foot and mouth disease and rinderpest.
Synonyms:
Extracted from bovine skin
References
1. Terato, K., et al. 'A rapid assay method of collagenase activity using 14C-labeled soluble collagen as substrate.' Biochim. Biophys. Acta 1976, 445, 753-762
2. Sellers, A., et al. 'Evidence that latent collagenases are enzyme-inhibitor complexes.' Biochem. J. 1977, 163, 303-307
3. Shinkai, H., et al. 'A complex of collagenase with low molecular weight inhibitors in the culture medium of embryonic chick skin explants.' J. Biochem. (Tokyo) 1977, 81, 261-263
4. Shinkai, H. & Nagai, Y., 'A latent collagenase from embryonic human skin explants.' J. Biochem. (Tokyo) 1977, 81, 1261-1263
5. Murawaki, Y., et al. 'Serum collagenase activity in patients with chronic liver disease.' J. Hepatol. 1993, 328-334
2. Sellers, A., et al. 'Evidence that latent collagenases are enzyme-inhibitor complexes.' Biochem. J. 1977, 163, 303-307
3. Shinkai, H., et al. 'A complex of collagenase with low molecular weight inhibitors in the culture medium of embryonic chick skin explants.' J. Biochem. (Tokyo) 1977, 81, 261-263
4. Shinkai, H. & Nagai, Y., 'A latent collagenase from embryonic human skin explants.' J. Biochem. (Tokyo) 1977, 81, 1261-1263
5. Murawaki, Y., et al. 'Serum collagenase activity in patients with chronic liver disease.' J. Hepatol. 1993, 328-334