Guanidine hydrochloride
| Code | Size | Price |
|---|
| CDX-G0040-G100 | 100 g | £59.00 |
Quantity:
| CDX-G0040-G500 | 500 g | £145.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+20°C
Documents
Further Information
Alternate Names/Synonyms:
Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride; Carbamimidoylazanium chloride
Appearance:
White powder.
CAS:
50-01-1
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS07
Handling Advice:
Protect from light and moisture.
Hazards:
H302+H332,H315,H319
InChi:
InChI=1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
InChiKey:
PJJJBBJSCAKJQF-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 50-01-1. Formula: CH5N3 . HCl. MW: 59.07 . 36.46. Guanidine hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. It is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins. At higher concentrations, this strong denaturant has been reported to solubilize denatured insoluble proteins such as inclusion bodies and to increase the solubility of hydrophobic molecules. When utilized at lower concentrations, it is useful in prompting the refolding of denatured proteins. Guanidine hydrochloride is also been reported to allow return of enzymatic activity in protocols using it as a first step. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
MDL:
MFCD00013026
Molecular Formula:
CH5N3 . HCl
Molecular Weight:
59.07 . 36.46
Package Type:
Vial
Precautions:
P261,P280,P301+P312+P330,P304+P340+P312,P305+P351+P338,P337+P313
Product Description:
Guanidine hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. It is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins. At higher concentrations, this strong denaturant has been reported to solubilize denatured insoluble proteins such as inclusion bodies and to increase the solubility of hydrophobic molecules. When utilized at lower concentrations, it is useful in prompting the refolding of denatured proteins. Guanidine hydrochloride is also been reported to allow return of enzymatic activity in protocols using it as a first step. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Purity:
>99% (Titration)
Signal word:
Warning
SMILES:
NC([NH3+])=N.[Cl-]
Solubility Chemicals:
Soluble in water (6M) or ethanol (1M).
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 2 years after receipt when stored at RT.
References
(1) C. Holm, et al.; Gene 42, 169 (1986) | (2) C.N. Pace, et al.; Biochemistry 29, 2564 (1990) | (3) F.A. Marston, et al.; Meth. Enzymol. 182, 264 (1990) | (4) R. Rudolph & H. Lilie; FASEB J. 10, 49 (1996) | (5) A. Mukhopadhyay; Adv. Biochem. Eng. Biotechnol. 56, 61 (1997) | (6) S. Emadi & M. Behzadi; BBRC 450, 1339 (2014)