Guanidine thiocyanate
| Code | Size | Price |
|---|
| CDX-G0058-G100 | 100 g | £78.00 |
Quantity:
| CDX-G0058-G500 | 500 g | £205.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+20°C
Documents
Further Information
Alternate Names/Synonyms:
Guanidinium rhodanide; GTC; Guanidinium isothiocyanate; Guanidinium thiocyanate
Appearance:
White to off-white powder.
CAS:
593-84-0
Class:
8
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS05,GHS07
Handling Advice:
Protect from light and moisture.
Hazards:
H302+H312+H332,H314,H412
InChi:
InChI=1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
InChiKey:
ZJYYHGLJYGJLLN-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 593-84-0. Formula: CH5N3 . CHNS. MW: 59.07 . 59.09. Guanidine thiocyanate is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. Guanidine thiocyanate is preferred for the purification of RNA because it dissociates the RNA into its nucleic acids and protein forms and is commonly used in the guanidinium thiocyanate-phenol-chloroform extraction method. As one of the strongest protein denaturants, it inactivates nucleases more than twice as fast as Guanidine hydrochloride. It is useful for denaturation and refolding of proteins. At high molarity solutions, Guanidine thiocyanate irreversibly inactivates RNase. Guanidine thiocyanate causes proteins to dissolve readily, thus disintegrating cellular structures.
MDL:
MFCD00013027
Molecular Formula:
CH5N3 . CHNS
Molecular Weight:
59.07 . 59.09
Package Type:
Vial
PG:
III
Precautions:
P260,P280,P301+P312+P330,P303+P361+P353,P304+P340+P310,P305+P351+P338
Product Description:
Guanidine thiocyanate is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. Guanidine thiocyanate is preferred for the purification of RNA because it dissociates the RNA into its nucleic acids and protein forms and is commonly used in the guanidinium thiocyanate-phenol-chloroform extraction method. As one of the strongest protein denaturants, it inactivates nucleases more than twice as fast as Guanidine hydrochloride. It is useful for denaturation and refolding of proteins. At high molarity solutions, Guanidine thiocyanate irreversibly inactivates RNase. Guanidine thiocyanate causes proteins to dissolve readily, thus disintegrating cellular structures.
Purity:
>99% (Titration)
Signal word:
Danger
SMILES:
NC(N)=N.SC#N
Solubility Chemicals:
Soluble in water (10M) or DMSO.
Transportation:
Excepted Quantity
UN Nummer:
1759
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 2 years after receipt when stored at RT.
References
(1) S. Lapanje; Biochim. Biophys. Acta 243, 349 (1971) | (2) S. Lapanje; Biochim. Biophys. Acta 243, 357 (1971) | (3) W.C. Johnson, et al.; Appl. Microbiol. 29, 405 (1975) | (4) R. McGookin; Methods Mol. Biol. 2, 113 (1985) | (5) P. Chomczynski, et al.; Anal. Biochem. 162, 156 (1987) | (6) T. Nakano & A.L. Fink; J. Biol. Chem. 265, 12356 (1990) | (7) S. Emadi & M. Behzadi; BBRC 450, 1339 (2014)