4-Methylumbelliferyl phosphate disodium salt
| Code | Size | Price |
|---|
| CDX-M0012-M250 | 250 mg | £65.00 |
Quantity:
| CDX-M0012-G001 | 1 g | £169.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+4°C
Documents
Further Information
Alternate Names/Synonyms:
4-MUP; 4-MU-phopahte; MUP; Disodium 4-methylumbelliferyl phosphate; 7-hydroxy-4-Methylcoumarin phosphate disodium salt
Appearance:
White to off-white powder.
CAS:
22919-26-2
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C10H9O6P.2Na/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;;/h2-5H,1H3,(H2,12,13,14);;/q;2*+1/p-2
InChiKey:
WUUDJQVNZPEPKN-UHFFFAOYSA-L
Long Description:
Chemical. CAS: 22919-26-2. Formula: C10H7Na2O6P. MW: 300.11. 4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate for phosphatases (mainly alkaline). It is converted to the fluorescent product 4-methylumbelliferone (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. Therefore, this phosphatase substrate is not well suited for living cell or continuous assays since 4-MU only develops maximum fluorescence at a pH value of >10. Spectral data: lambdaex=319nm; lambdaem=384nm (pH 9.0). lambdaex=360nm; lambdaem=449nm (for reaction product).
MDL:
MFCD00037614
Molecular Formula:
C10H7Na2O6P
Molecular Weight:
300.11
Package Type:
Vial
Product Description:
4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate for phosphatases (mainly alkaline). It is converted to the fluorescent product 4-methylumbelliferone (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. Therefore, this phosphatase substrate is not well suited for living cell or continuous assays since 4-MU only develops maximum fluorescence at a pH value of >10. Spectral data: lambdaex=319nm; lambdaem=384nm (pH 9.0). lambdaex=360nm; lambdaem=449nm (for reaction product).
Purity:
>97% (NMR)
SMILES:
CC(C1=CC=C(OP([O-])([O-])=O)C=C1O2)=CC2=O.[Na+].[Na+]
Solubility Chemicals:
Soluble in water (50 mg/ml).
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) D. Robinson & P. Willcox; Biochim. Biophys. Acta 191, 183 (1969) | (2) C.J. Cornish, et al.; Am. J. Clin. Pathol. 53, 68 (1970) | (3) J.P. Chambers, et al.; Clin. Chim. Acta 80, 67 (1977) | (4) F. Watanabe, et al.; Clin. Chim. Acta 91, 273 (1979) | (5) F.A. Anthony, et al.; Anal. Biochem. 155, 103 (1986) | (6) K. Soyama, et al.; Forensic Sci. Int. 18, 195 (1981) | (7) H.J. Radzun, et al.; Clin. Chim. Acta 123, 203 (1982) | (8) F.A. Anthony, et al.; Anal. Biochem. 155, 103 (1986) | (9) K.Y. Xiao, et al.; J. Tongji Med. Univ. 13, 206 (1993) | (10) K. Bednarska, et al.; Mediators Inflamm. 2006, 19307 (2006) | (11) J.J. Weiland, et al.; Anal. Biochem. 361, 140 (2007)