Thiazolyl blue tetrazolium bromide
| Code | Size | Price |
|---|
| CDX-T0186-G001 | 1 g | £65.00 |
Quantity:
| CDX-T0186-G005 | 5 g | £230.00 |
Quantity:
| CDX-T0186-G010 | 10 g | £401.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+4°C
Documents
Further Information
Alternate Names/Synonyms:
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; MTT; Methylthiazolyldiphenyl-tetrazolium bromide; NSC 60102
Appearance:
Yellow powder.
CAS:
298-93-1
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS07,GHS08
Handling Advice:
Protect from light and moisture.
Hazards:
H315,H319,H335,H341
InChi:
InChI=1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
InChiKey:
AZKSAVLVSZKNRD-UHFFFAOYSA-M
Long Description:
Chemical. CAS: 298-93-1. Formula: C18H16BrN5S. MW: 414.32. MTT is a common histochemical/cytochemical reagent and for the detection of NAD. MTT is used to detect reductive metabolism in cells. It is a cell-permeable yellow dye that is reduced by intracellular NAD(P)H-oxidoreductases in mitochondria of living cells to form the dark blue water-insoluble MTT-formazan. The intensity of the color can then be measured spectrophotometrically at 540 or 570nm, which allows quantitation of cell viability and cell proliferation. It is frequently used in colorimetric assays to measure cell proliferation/cell growth, cytotoxicity (such as for screening cancer drugs), and apoptosis.
MDL:
MFCD00011964
Molecular Formula:
C18H16BrN5S
Molecular Weight:
414.32
Package Type:
Vial
Precautions:
P261,P281,P305+P351+P338
Product Description:
MTT is a common histochemical/cytochemical reagent and for the detection of NAD. MTT is used to detect reductive metabolism in cells. It is a cell-permeable yellow dye that is reduced by intracellular NAD(P)H-oxidoreductases in mitochondria of living cells to form the dark blue water-insoluble MTT-formazan. The intensity of the color can then be measured spectrophotometrically at 540 or 570nm, which allows quantitation of cell viability and cell proliferation. It is frequently used in colorimetric assays to measure cell proliferation/cell growth, cytotoxicity (such as for screening cancer drugs), and apoptosis.
Purity:
>98% (HPLC)
Signal word:
Warning
SMILES:
CC1=C(C)SC([N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=CC=C4)=N1.[Br-]
Solubility Chemicals:
Soluble in water (5mg/ml).
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.
References
(1) M.A. Abdallah, & J.-F. Biellmann; Eur. J. Biochem. 112, 331 (1980) | (2) Y. Liu, et al.; J. Neurochem. 69, 581 (1997) | (3) M.V. Berridge, et al.; Biotechnol. Ann. Rev. 11, 127 (2005) | (4) T. Hayon, et al.; Leuk. Lymphoma 44, 1957 (2003) (Review) | (5) K. Praebst, et al.; Methods Mol. Biol. 1601, 1 (2017) | (6) P. Kumar, et al.; Cold Spring Harb. Protoc. 2018, pdb.prot095505 (2018) | (7) E. Grela, et al.; Acta Histochem. 120, 303 (2018) (Review)