APAF1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4704
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4704-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: BHF-1
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the APAF1 antibody at -20°C.

Images

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Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with APAF1 antibody. Predicted molecular weight ~140 kDa.
2 / 2
IHC staining of FFPE mouse kidney tissue with APAF1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with APAF1 antibody. Predicted molecular weight ~140 kDa.
IHC staining of FFPE mouse kidney tissue with APAF1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the APAF1 antibody should be determined by the researcher.
Description:
Apoptotic Peptidase Activating Factor 1 is a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis.
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Apoptotic Peptidase Activating Factor 1 was used as the immunogen for the APAF1 antibody.
Limitation:
This APAF1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
O14727