KLF4 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4737
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4737-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: AODA-11
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store the KLF4 antibody at -20°C.

Images

1 / 3
Western blot testing of 1) human HeLa, 2) mouse NIH3T3 and 3) rat C6 cell lysate with KLF4 antibody. Predicted molecular weight: 50-60 kDa + possible ~75 kDa (phosphorylated form).
2 / 3
IHC staining of FFPE mouse liver tissue with KLF4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Immunofluorescent staining of FFPE human HeLa cells with KLF4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of 1) human HeLa, 2) mouse NIH3T3 and 3) rat C6 cell lysate with KLF4 antibody. Predicted molecular weight: 50-60 kDa + possible ~75 kDa (phosphorylated form).
IHC staining of FFPE mouse liver tissue with KLF4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with KLF4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the KLF4 antibody should be determined by the researcher.
Description:
The KLF4 gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human KLF4 was used as the immunogen for the KLF4 antibody.
Limitation:
This KLF4 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O43474