Brd4 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4766
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4766-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: DBH-2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the Brd4 antibody at -20°C.

Images

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Western blot testing of human HeLa cell lysate with TAX1BP3 antibody. Predicted molecular weight ~156 kDa (long form) and ~81 kDa (short form).
2 / 3
IHC staining of FFPE human kidney tissue with Brd4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
Immunofluorescent staining of FFPE human HeLa cells with Brd4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of human HeLa cell lysate with TAX1BP3 antibody. Predicted molecular weight ~156 kDa (long form) and ~81 kDa (short form).
IHC staining of FFPE human kidney tissue with Brd4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with Brd4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence/Immunocytochemistry: 1:50-1:200
Application Note:
Optimal dilution of the Brd4 antibody should be determined by the researcher.
Description:
Bromodomain-containing protein 4 is a chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation.
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Brd4 was used as the immunogen for the Brd4 antibody.
Limitation:
This Brd4 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
O60885