IRE1 Antibody (pS724)

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NSJ Bioreagents
Product Code: NSJ-RQ4781
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4781-100ul100ul£535.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: EGH-5
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store the phospho-IRE1 antibody at -20°C.

Images

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Western blot testing of lysate from 1) untreated and 2) AP-treated human K562 cells with phospho-IRE1 antibody. Predicted molecular weight ~110 kDa.
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Western blot testing of lysate from 1) untreated and 2) AP-treated human K562 cells with phospho-IRE1 antibody. Predicted molecular weight ~110 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000
Application Note:
Optimal dilution of the phospho-IRE1 antibody should be determined by the researcher.
Description:
IRE1 is a serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the ER luminal domain is maintained in its inactive monomeric state by binding to the ER chaperone HSPA5/BiP. Accumulation of misfolded protein in the ER causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. [UniProt]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human IRE1 / ERN1 (surrounding pS724) was used as the immunogen for the phospho-IRE1 antibody.
Limitation:
This phospho-IRE1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
O75460