ATG12 Antibody

NSJ Bioreagents
Product Code: NSJ-RQ4798
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4798-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: ABID-1
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store the ATG12 antibody at -20°C.

Images

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Western blot testing of human HepG2 lysate with ATG12 antibody. Expected molecular weight: ~15 kDa (ATG12) and 50-60 kDa (ATG12-ATG5 complex).

Western blot testing of human HepG2 lysate with ATG12 antibody. Expected molecular weight: ~15 kDa (ATG12) and 50-60 kDa (ATG12-ATG5 complex).

Further Information

Application Details :
Western blot: 1:500-1:2000
Application Note:
Optimal dilution of the ATG12 antibody should be determined by the researcher.
Description:
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. [UniProt]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human ATG12 was used as the immunogen for the ATG12 antibody.
Limitation:
This ATG12 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
O94817