EGFR Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4838
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4838-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: AEF-5
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the EGFR antibody at -20°C.

Images

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Western blot testing of human HeLa cell lysate with EGFR antibody. Expected molecular weight: 134-170 kDa depending on glycosylation level.
2 / 2
IHC staining of FFPE human stomach cancer with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of human HeLa cell lysate with EGFR antibody. Expected molecular weight: 134-170 kDa depending on glycosylation level.
IHC staining of FFPE human stomach cancer with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:5000-1:10000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the EGFR antibody should be determined by the researcher.
Description:
EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human EGFR was used as the immunogen for the EGFR antibody.
Limitation:
This EGFR antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P00533