c-Myc Antibody

NSJ Bioreagents
Product Code: NSJ-RQ4860
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4860-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: AHI-13
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the c-Myc antibody at -20°C.

Images

1 / 3
IF/ICC staining of human HeLa cells with c-Myc antibody (green) and DAPI nuclear stain (blue).
2 / 3
IHC staining of FFPE human lung cancer with c-Myc antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
Western blot testing of human 1) Jurkat and 2) K562 cell lysate with c-Myc antibody. Theoretical molecular weight: ~50 kDa but routinely observed at 50~70 kDa.

IF/ICC staining of human HeLa cells with c-Myc antibody (green) and DAPI nuclear stain (blue).
IHC staining of FFPE human lung cancer with c-Myc antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human 1) Jurkat and 2) K562 cell lysate with c-Myc antibody. Theoretical molecular weight: ~50 kDa but routinely observed at 50~70 kDa.

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence/Immunocytochemistry: 1:50-1:200
Application Note:
Optimal dilution of the c-Myc antibody should be determined by the researcher.
Description:
Myc is a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors.
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human c-Myc / MYC was used as the immunogen for the c-Myc antibody.
Limitation:
This c-Myc antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P01106