tPA Antibody / Tissue Plasminogen Activator

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NSJ Bioreagents
Product Code: NSJ-RQ4883
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4883-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Antibody Clone: n/a
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
After reconstitution, the tPA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human HeLa, 2) human U-2 OS, 3) human U-87 MG, 4) rat liver, 5) rat kidney, 6) mouse liver and 7) mouse kidney lysate with tPA antibody at 0.5ug/ml. Expected molecular weight: 64-70 kDa.
2 / 3
IHC staining of FFPE human placental tissue with tPA antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
Flow cytometry testing of human U-251 cells with tPA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= tPA antibody.
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Western blot testing of 1) human HeLa, 2) human U-2 OS, 3) human U-87 MG, 4) rat liver, 5) rat kidney, 6) mouse liver and 7) mouse kidney lysate with tPA antibody at 0.5ug/ml. Expected molecular weight: 64-70 kDa.
IHC staining of FFPE human placental tissue with tPA antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human U-251 cells with tPA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= tPA antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow Cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the tPA antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
PLAT is also known as tPA. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QKDVPGVYTKVTNYLDWIRDNMRP from the human protein were used as the immunogen for the tPA antibody.
Limitation:
This tPA antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P00750