Annexin A3 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4892
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4892-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Antibody Clone: n/a
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
After reconstitution, the Annexin A3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human lung cancer with Annexin A3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human intestinal cancer with Annexin A3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human breast cancer with Annexin A3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 6
Western blot testing of 1) rat lung, 2) rat ovary, 3) mouse lung and 4) mouse ovary with Annexin A3 antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.
5 / 6
IHC staining of FFPE mouse kidney with Annexin A3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 6
IHC staining of FFPE rat kidney with Annexin A3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer with Annexin A3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with Annexin A3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with Annexin A3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of 1) rat lung, 2) rat ovary, 3) mouse lung and 4) mouse ovary with Annexin A3 antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.
IHC staining of FFPE mouse kidney with Annexin A3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat kidney with Annexin A3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Annexin A3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Annexin A3 is a protein that in humans is encoded by the Annexin A3 gene. The Annexin A3 gene contains 13 exons and spans 58 kb of genomic DNA. The Annexin A3 gene is mapped to 4q21. It is abnormally expressed in fetuses of both IVF and ICSI, which may contribute to the increase risk of birth defects in these ART. This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phospholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids D23-D323 from the human protein were used as the immunogen for the Annexin A3 antibody.
Limitation:
This Annexin A3 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P12429