Wee1 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4901
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4901-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Antibody Clone: n/a
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
After reconstitution, the Wee1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human breast carcinoma with Wee1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
2 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with Wee1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 4
Western blot testing of human 1) HeLa, 2) placenta, 3) A431 and 4) U-2 OS cel lysate with Wee1 antibody at 0.5ug/ml. Predicted molecular weight ~72 kDa.
4 / 4
Flow cytometry testing of human SiHa cells with Wee1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Wee1 antibody.
❮❯

IHC staining of FFPE human breast carcinoma with Wee1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with Wee1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) placenta, 3) A431 and 4) U-2 OS cel lysate with Wee1 antibody at 0.5ug/ml. Predicted molecular weight ~72 kDa.
Flow cytometry testing of human SiHa cells with Wee1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Wee1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Wee1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Wee1 is a nuclear kinase belonging to the Ser/Thr family of protein kinases in the fission yeast Schizosaccharomyces pombe (S. pombe). I t is mapped to 11p15.4. This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids ELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDR from the human protein were used as the immunogen for the Wee1 antibody.
Limitation:
This Wee1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P30291