SF3B4 Antibody / Splicing factor 3B subunit 4

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NSJ Bioreagents
Product Code: NSJ-RQ4925
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4925-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Antibody Clone: n/a
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the SF3B4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) placenta, 2) MDA-MB-231, 3) U-2 OS, 4) HL-60, 5) A549, 6) PC-3, 7) ThP-1 and 8) U-87 MG lysate with SF3B4 antibody at 0.5ug/ml. Predicted molecular weight ~44 kDa but routinely observed at ~49 kDa.
2 / 2
Western blot testing of 1) rat brain, 2) rat lung, 3) rat liver, 4) rat stomach, 5) mouse brain, 6) mouse lung, 7) mouse liver, 8) mouse stomach and 9) mouse NIH3T3 lysate with SF3B4 antibody at 0.5ug/ml. Predicted molecular weight ~44 kDa but routinely observed at ~49 kDa.
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Western blot testing of human 1) placenta, 2) MDA-MB-231, 3) U-2 OS, 4) HL-60, 5) A549, 6) PC-3, 7) ThP-1 and 8) U-87 MG lysate with SF3B4 antibody at 0.5ug/ml. Predicted molecular weight ~44 kDa but routinely observed at ~49 kDa.
Western blot testing of 1) rat brain, 2) rat lung, 3) rat liver, 4) rat stomach, 5) mouse brain, 6) mouse lung, 7) mouse liver, 8) mouse stomach and 9) mouse NIH3T3 lysate with SF3B4 antibody at 0.5ug/ml. Predicted molecular weight ~44 kDa but routinely observed at ~49 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
Optimal dilution of the SF3B4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Splicing factor 3B subunit 4 is a protein that in humans is encoded by the SF3B4 gene. This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids LDEKVSEPLLWELFLQA from the human protein were used as the immunogen for the SF3B4 antibody.
Limitation:
This SF3B4 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15427