RAB11B Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4926
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4926-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Antibody Clone: n/a
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
After reconstitution, the RAB11B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of human 1) placenta, 2) A549, 3) U-87 MG, 4) PC-3, 5) HeLa, 6) Caco-2 and 7) HL-60 lysate with RAB11B antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
2 / 7
Western blot testing of 1) rat spleen, 2) rat lung, 3) rat ovary, 4) rat kidney, 5) mouse lung, 6) mouse ovary, 7) mouse kidney and 8) mouse SP2/0 lysate with RAB11B antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
3 / 7
IHC staining of FFPE human esophageal squamous cancer with RAB11B antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human ovarian cancer with RAB11B antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 7
Flow cytometry testing of human A549 cells with RAB11B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB11B antibody.
6 / 7
IHC staining of FFPE mouse ovary with RAB11B antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
7 / 7
IHC staining of FFPE rat ovary with RAB11B antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of human 1) placenta, 2) A549, 3) U-87 MG, 4) PC-3, 5) HeLa, 6) Caco-2 and 7) HL-60 lysate with RAB11B antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
Western blot testing of 1) rat spleen, 2) rat lung, 3) rat ovary, 4) rat kidney, 5) mouse lung, 6) mouse ovary, 7) mouse kidney and 8) mouse SP2/0 lysate with RAB11B antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
IHC staining of FFPE human esophageal squamous cancer with RAB11B antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with RAB11B antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human A549 cells with RAB11B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB11B antibody.
IHC staining of FFPE mouse ovary with RAB11B antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat ovary with RAB11B antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow Cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the RAB11B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Ras-related protein Rab-11B is a protein that in humans is encoded by the RAB11B gene. It is mapped to 19p13.2. The Ras superfamily of small GTP-binding proteins, which includes the Ras, Ral, Rho, Rap, and Rab families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QKQIADRAAHDESPGNNVVDISVPPTTDGQKPNKLQCC from the human protein were used as the immunogen for the RAB11B antibody.
Limitation:
This RAB11B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15907