DOK7 Antibody / Docking protein 7

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4928
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4928-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Antibody Clone: n/a
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the DOK7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 2
Western blot testing of 1) human HeLa, 2) human PC-3, 3) human HL-60, 4) rat skeletal muscle, 5) rat heart, 6) mouse skeletal muscle and 7) mouse heart lysate with DOK7 antibody at 0.5ug/ml. Predicted molecular weight ~53 kDa, observed here at ~65 kDa.
2 / 2
IF/ICC staining of FFPE human A431 cells with DOK7 antibody at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
❮❯

Western blot testing of 1) human HeLa, 2) human PC-3, 3) human HL-60, 4) rat skeletal muscle, 5) rat heart, 6) mouse skeletal muscle and 7) mouse heart lysate with DOK7 antibody at 0.5ug/ml. Predicted molecular weight ~53 kDa, observed here at ~65 kDa.
IF/ICC staining of FFPE human A431 cells with DOK7 antibody at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunocytochemistry/Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the DOK7 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Dok-7 is a non-catalytic cytoplasmic adaptor protein that is expressed specifically in muscle and is essential for the formation of neuromuscular synapses. Further, Dok-7 contains pleckstrin homology (PH) and phosphotyrosine-binding (PTB) domains that are critical for Dok-7 function. It is mapped to 4p16.3. The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids STVEERVAQEALETLQLEKRLSLLSHAGR from the human protein were used as the immunogen for the DOK7 antibody.
Limitation:
This DOK7 antibody is available for research use only.
Localization:
Cell membrane, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q18PE1