TIMM17A Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4949
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4949-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Antibody Clone: n/a
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
After reconstitution, the TIMM17A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 10
Western blot testing of human 1) placenta, 2) K562, 3) T-47D, 4) Caco-2, 5) HeLa, 6) ThP-1, 7) A431 and 8) A549 lysate with TIMM17A antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
2 / 10
Western blot testing of 1) rat heart, 2) rat lung, 3) rat liver, 4) rat brain, 5) mouse heart, 6) mouse lung, 7) mouse liver, 8) mouse brain and 9) mouse Neuro-2a lysate with TIMM17A antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
3 / 10
IHC staining of FFPE human breast cancer with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human liver cancer with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human lung cancer with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE rat lung with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
7 / 10
Flow cytometry testing of human A431 cells with TIMM17A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TIMM17A antibody.
8 / 10
IHC staining of FFPE mouse lung tissue with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
9 / 10
IF/ICC staining of FFPE human A549 cells with TIMM17A antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 10
IF/ICC staining of FFPE human HeLa cells with TIMM17A antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human 1) placenta, 2) K562, 3) T-47D, 4) Caco-2, 5) HeLa, 6) ThP-1, 7) A431 and 8) A549 lysate with TIMM17A antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
Western blot testing of 1) rat heart, 2) rat lung, 3) rat liver, 4) rat brain, 5) mouse heart, 6) mouse lung, 7) mouse liver, 8) mouse brain and 9) mouse Neuro-2a lysate with TIMM17A antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
IHC staining of FFPE human breast cancer with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat lung with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with TIMM17A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TIMM17A antibody.
IHC staining of FFPE mouse lung tissue with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IF/ICC staining of FFPE human A549 cells with TIMM17A antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IF/ICC staining of FFPE human HeLa cells with TIMM17A antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow Cytometry: 1-3ug/million cells, Immunofluorescence/Immunocytochemistry: 2-4ug/ml
Application Note:
Optimal dilution of the TIMM17A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Mitochondrial import inner membrane translocase subunit Tim17-A is an enzyme that in humans is encoded by the TIMM17A gene. It is mapped to 1q32.1. TIM17 is a conserved suppressor of mtDNA instability, and overexpression of TIMM17A can prevent mtDNA loss in a human cellular model of mitochondrial disease.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids LFSMIDCSMVQVRGKED from the human protein were used as the immunogen for the TIMM17A antibody.
Limitation:
This TIMM17A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q99595