SRCIN1 Antibody
Code | Size | Price |
---|
NSJ-RQ4950-100ug | 100ug | £535.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Antibody Clone: n/a
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
After reconstitution, the SRCIN1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Images
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Further Information
Application Details :
Flow cytometry: 1-3ug/million cells,Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SRCIN1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Using yeast 2-hybrid analysis, protein pull-down assays, and mutation analysis, it is showed that the first coiled-coil domain of rat Snip interacted with the N-terminal t-SNARE domain of Snap25 (600322). Biochemical studies demonstrated that Snip was tightly associated with rat brain cytoskeleton. Indirect immunofluorescence and confocal microscopy of rat PC12 pheochromocytoma cells revealed colocalization of Snip with Snap25 in the actin cytoskeleton, particularly in filopodia, lamellipodia, and neuritic extensions, including the tips. Overexpression of Snip or its Snap25-interacting domain inhibited calcium-dependent exocytosis from PC12 cells. It is concluded that SNIP is involved in regulation of neurosecretion, perhaps via its interaction with SNAP25 and the cytoskeleton.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids E189-E287 from the human protein were used as the immunogen for the SRCIN1 antibody.
Limitation:
This SRCIN1 antibody is available for research use only.
Localization:
Cytoplasm, plasma membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9C0H9