PPCS Antibody / Phosphopantothenoylcysteine synthetase

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NSJ Bioreagents
Product Code: NSJ-RQ4952
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4952-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Antibody Clone: n/a
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
After reconstitution, the PPCS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) placenta and 3) PC-3 lysate with PPCS antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.
2 / 3
IHC staining of FFPE human intestinal cancer with PPCS antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
Flow cytometry testing of human A431 cells with PPCS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPCS antibody.
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Western blot testing of human 1) HeLa, 2) placenta and 3) PC-3 lysate with PPCS antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.
IHC staining of FFPE human intestinal cancer with PPCS antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with PPCS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPCS antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow Cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PPCS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Phosphopantothenoylcysteine synthetase in humans is encoded by the PPCS gene. Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. By genomic sequence analysis, this PPCS gene ia mapped to chromosome 1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids VEIEEKIVDNLQSRHTAFIGDRN from the human protein were used as the immunogen for the PPCS antibody.
Limitation:
This PPCS antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9HAB8