HDAC5 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4959
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4959-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Antibody Clone: n/a
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
After reconstitution, the HDAC5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of human 1) HeLa, 2) placenta, 3) ThP-1, 4) K562, 5) PC-3 and 6) Caco-2 lysate with HDAC5 antibody at 0.5ug/ml. Predicted molecular weight ~122 kDa, observed at 122-160 kDa.
2 / 5
Western blot testing of 1) rat heart, 2) rat liver, 3) rat stomach, 4) rat ovary, 5) mouse heart, 6) mouse liver, 7) mouse stomach, 8) mouse ovary and 9) mouse NIH3T3 lysate with HDAC5 antibody at 0.5ug/ml. Predicted molecular weight ~122 kDa, observed at 122-160 kDa.
3 / 5
IHC staining of FFPE human placental tissue with HDAC5 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE mouse intestine with HDAC5 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 5
IHC staining of FFPE rat intestine with HDAC5 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of human 1) HeLa, 2) placenta, 3) ThP-1, 4) K562, 5) PC-3 and 6) Caco-2 lysate with HDAC5 antibody at 0.5ug/ml. Predicted molecular weight ~122 kDa, observed at 122-160 kDa.
Western blot testing of 1) rat heart, 2) rat liver, 3) rat stomach, 4) rat ovary, 5) mouse heart, 6) mouse liver, 7) mouse stomach, 8) mouse ovary and 9) mouse NIH3T3 lysate with HDAC5 antibody at 0.5ug/ml. Predicted molecular weight ~122 kDa, observed at 122-160 kDa.
IHC staining of FFPE human placental tissue with HDAC5 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with HDAC5 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with HDAC5 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the HDAC5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Histone deacetylase 5, also called HDAC5 or KIAA0600, is an enzyme that in humans is encodes by the HDAC5 gene. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. This gene is mapped to 17q21.31. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids H102-E197 from the human protein were used as the immunogen for the HDAC5 antibody.
Limitation:
This HDAC5 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UQL6