Estrogen Receptor alpha Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5022
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5022-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: IE-5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the ER alpha antibody at -20°C.

Images

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Western blot testing of human 1) MCF7 and 2) T-47D cell lysate with ER alpha antibody. Predicted molecular weight ~66 kDa.
2 / 2
IHC staining of FFPE human cervical carcinoma with ER alpha antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of human 1) MCF7 and 2) T-47D cell lysate with ER alpha antibody. Predicted molecular weight ~66 kDa.
IHC staining of FFPE human cervical carcinoma with ER alpha antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
Optimal dilution of the ER alpha antibody should be determined by the researcher.
Description:
Estrogen receptor alpha is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human ER alpha / ESR1 was used as the immunogen for the ER alpha antibody.
Limitation:
This ER alpha antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P03372