GAPDH Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5052
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5052-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: BH-7
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the GAPDH antibody at -20°C.

Images

1 / 6
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) mouse kidney, 4) mouse spleen, 5) mouse RAW264 and 6) rat brain lysate with GAPDH antibody. Predicted molecular weight ~36 kDa.
2 / 6
IF/ICC staining of human HeLa cells with GAPDH antibody (green) and DAPI nuclear stain (blue).
3 / 6
IHC staining of FFPE human uterus with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human kidney with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 6
IHC staining of FFPE mouse testis with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 6
IHC staining of FFPE rat colon with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of 1) human HeLa, 2) human Jurkat, 3) mouse kidney, 4) mouse spleen, 5) mouse RAW264 and 6) rat brain lysate with GAPDH antibody. Predicted molecular weight ~36 kDa.
IF/ICC staining of human HeLa cells with GAPDH antibody (green) and DAPI nuclear stain (blue).
IHC staining of FFPE human uterus with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human kidney with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse testis with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat colon with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:3000-1:10000,Immunohistochemistry (FFPE): 1:100-1:500,Immunofluorescence/Immunocytochemistry: 1:100-1:250
Application Note:
Optimal dilution of the GAPDH antibody should be determined by the researcher.
Description:
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human GAPDH was used as the immunogen for the GAPDH antibody.
Limitation:
This GAPDH antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P04406