Hydroxyl-Histone H2A Antibody (Tyr39)

NSJ Bioreagents
Product Code: NSJ-RQ5086
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5086-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: DED-8
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the hydroxyl-Histone H2A antibody (Tyr39) at -20°C.

Images

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Western blot testing of 1) mouse NIH3T3 and 2) human A549 cell lysate with hydroxyl-Histone H2A antibody (Tyr39). Predicted molecular weight ~14 kDa.
2 / 2
IHC staining of FFPE mouse colon tissue with hydroxyl-Histone H2A antibody (Tyr39). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Western blot testing of 1) mouse NIH3T3 and 2) human A549 cell lysate with hydroxyl-Histone H2A antibody (Tyr39). Predicted molecular weight ~14 kDa.
IHC staining of FFPE mouse colon tissue with hydroxyl-Histone H2A antibody (Tyr39). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:500-1:2000
Application Note:
Optimal dilution of the hydroxyl-Histone H2A antibody (Tyr39) should be determined by the researcher.
Description:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [UniProt]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Histone H2A (surrounding hydroxylated tyrosine 39) was used as the immunogen for the hydroxyl-Histone H2A antibody.
Limitation:
This hydroxyl-Histone H2A antibody (Tyr39) is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P04908