Tissue-nonspecific Alkaline Phosphatase Antibody / ALPL

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NSJ Bioreagents
Product Code: NSJ-RQ5119
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5119-100ul100ul£535.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: DBF-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the ALPL antibody at -20°C.

Images

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Western blot testing of human 1) HepG2 and 2) JAr cell lysate with ALPL antibody. Predicted molecular weight ~57 kDa, but can be observed at up to ~75 kDa due to glycosylation.
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IHC staining of FFPE human liver tissue with ALPL antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of human 1) HepG2 and 2) JAr cell lysate with ALPL antibody. Predicted molecular weight ~57 kDa, but can be observed at up to ~75 kDa due to glycosylation.
IHC staining of FFPE human liver tissue with ALPL antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:5000-1:10000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the ALPL antibody should be determined by the researcher.
Description:
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Alkaline Phosphatase / ALPL was used as the immunogen for the ALPL antibody.
Limitation:
This ALPL antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P05186