Phospho-EIF2S1 Antibody (pS51)

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NSJ Bioreagents
Product Code: NSJ-RQ5120
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5120-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: IO-5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the phospho-EIF2S1 antibody (pS51) at -20°C.

Images

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Western blot testing of lysate from human HeLa cells 1) treated with Calyculin A and 2) untreated, with phospho-EIF2S1 antibody (pS51). Predicted molecular weight ~36 kDa.
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IHC staining of FFPE human colon cancer with phospho-EIF2S1 antibody (pS51). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of lysate from human HeLa cells 1) treated with Calyculin A and 2) untreated, with phospho-EIF2S1 antibody (pS51). Predicted molecular weight ~36 kDa.
IHC staining of FFPE human colon cancer with phospho-EIF2S1 antibody (pS51). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the phospho-EIF2S1 antibody (pS51) should be determined by the researcher.
Description:
EIF2S1 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. [UniProt]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human EIF2S1 (surrounding phosphorylated S51) was used as the immunogen for the phospho-EIF2S1 antibody.
Limitation:
This phospho-EIF2S1 antibody (pS51) is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P05198