Cytokeratin 18 Antibody / KRT18

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NSJ Bioreagents
Product Code: NSJ-RQ5131
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5131-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: GBF-11
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the CK18 antibody at -20°C.

Images

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IHC staining of FFPE mouse liver tissue with CK18 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 3
IF/ICC staining of rat PC-12 cells with CK18 antibody (green) and DAP nuclear stain (blue).
3 / 3
Western blot testing of human HeLa cell lysate with CK18 antibody. Predicted molecular weight ~48 kDa.
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IHC staining of FFPE mouse liver tissue with CK18 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IF/ICC staining of rat PC-12 cells with CK18 antibody (green) and DAP nuclear stain (blue).
Western blot testing of human HeLa cell lysate with CK18 antibody. Predicted molecular weight ~48 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:100-1:500,Immunofluorescence/Immunocytochemistry: 1:50-1:200
Application Note:
Optimal dilution of the CK18 antibody should be determined by the researcher.
Description:
KRT18 encodes the type I intermediate filament chain keratin 18. Cytokeratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Cytokeratin 18 / KRT18 was used as the immunogen for the CK18 antibody.
Limitation:
This CK18 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P05784