Phospho-CDK1/2 Antibody (pT14)

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ5152
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5152-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: DFH-3
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the phospho-CDK1/2 antibody (pT14) at -20°C.

Images

1 / 2
Western blot testing of lysate from human HeLa cells treated with hydroxyurea, with phospho-CDK1/2 antibody (pT14). Predicted molecular weight ~33 kDa.
2 / 2
IHC staining of FFPE rat spleen tissue with phospho-CDK1/2 antibody (pT14). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
❮❯

Western blot testing of lysate from human HeLa cells treated with hydroxyurea, with phospho-CDK1/2 antibody (pT14). Predicted molecular weight ~33 kDa.
IHC staining of FFPE rat spleen tissue with phospho-CDK1/2 antibody (pT14). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the phospho-CDK1/2 antibody (pT14) should be determined by the researcher.
Description:
Cyclin-dependent kinases (CDKs) are a family of protein kinases first discovered for their role in regulating the cell cycle. They are also involved in regulating transcription, mRNA processing, and the differentiation of nerve cells.[1] They are present in all known eukaryotes, and their regulatory function in the cell cycle has been evolutionarily conserved. [Wiki]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human CDK1/2 (surrounding phosphorylated T14) was used as the immunogen for the phospho-CDK1/2 antibody.
Limitation:
This phospho-CDK1/2 antibody (pT14) is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P06493