Alpha Enolase Antibody / ENO1

NSJ Bioreagents
Product Code: NSJ-RQ5160
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5160-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: AEA-5
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the Alpha Enolase antibody at -20°C.

Images

1 / 2
Western blot testing of 1) human MCF7 and 2) rat brain lysate with Alpha Enolase antibody. Predicted molecular weight ~47 kDa.
2 / 2
IHC staining of FFPE human colon tissue with Alpha Enolase antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Western blot testing of 1) human MCF7 and 2) rat brain lysate with Alpha Enolase antibody. Predicted molecular weight ~47 kDa.
IHC staining of FFPE human colon tissue with Alpha Enolase antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the Alpha Enolase antibody should be determined by the researcher.
Description:
The ENO1 gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Alpha Enolase / ENO1 was used as the immunogen for the Alpha Enolase antibody.
Limitation:
This Alpha Enolase antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P06733