Beta Tubulin Antibody / TUBB

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NSJ Bioreagents
Product Code: NSJ-RQ5179
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5179-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: BI-20
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the Beta Tubulin antibody at -20°C.

Images

1 / 6
IF/ICC staining of mouse NIH3T3 cells with Beta Tubulin antibody (red), PCNA antibody (green) and DAPI nuclear stain (blue).
2 / 6
Western blot testing of 1) human MCF7, 2) monkey COS-1, 3) human Jurkat and 4) human HeLa lysate with Beta Tubulin antibody. Predicted molecular weight: 50-55 kDa.
3 / 6
IHC staining of FFPE human uterine cancer with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human glioma with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 6
IHC staining of FFPE mouse kidney with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 6
IHC staining of FFPE rat brain with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IF/ICC staining of mouse NIH3T3 cells with Beta Tubulin antibody (red), PCNA antibody (green) and DAPI nuclear stain (blue).
Western blot testing of 1) human MCF7, 2) monkey COS-1, 3) human Jurkat and 4) human HeLa lysate with Beta Tubulin antibody. Predicted molecular weight: 50-55 kDa.
IHC staining of FFPE human uterine cancer with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human glioma with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat brain with Beta Tubulin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:3000-1:10000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence/Immunocytochemistry: 1:50-1:200
Application Note:
Optimal dilution of the Beta Tubulin antibody should be determined by the researcher.
Description:
The TUBB gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Beta Tubulin / TUBB was used as the immunogen for the Beta Tubulin antibody.
Limitation:
This Beta Tubulin antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P07437