MET Antibody / c-Met / HGFR

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NSJ Bioreagents
Product Code: NSJ-RQ5243
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5243-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: BFA-13
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the c-Met antibody at -20°C.

Images

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IHC staining of FFPE human stomach tissue with c-Met antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 3
Western blot testing of human 293 cell lysate with c-Met antibody. Predicted molecular weight ~156 kDa.
3 / 3
Immunofluorescent staining of FFPE human HT-29 cells with c-Met antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC staining of FFPE human stomach tissue with c-Met antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human 293 cell lysate with c-Met antibody. Predicted molecular weight ~156 kDa.
Immunofluorescent staining of FFPE human HT-29 cells with c-Met antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence: 1:50-1:200
Application Note:
Optimal dilution of the c-Met antibody should be determined by the researcher.
Description:
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human c-Met / MET was used as the immunogen for the c-Met antibody.
Limitation:
This c-Met antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P08581