Vimentin Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5250
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5250-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: AGF-22
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the VIM antibody at -20°C.

Images

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IHC staining of FFPE human colon tissue with VIM antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 2
Western blot testing of human HEK293 cell lysate with VIM antibody. Expected molecular weight: 53-58 kDa.
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IHC staining of FFPE human colon tissue with VIM antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human HEK293 cell lysate with VIM antibody. Expected molecular weight: 53-58 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the VIM antibody should be determined by the researcher.
Description:
The VIM gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Vimentin / VIM was used as the immunogen for the VIM antibody.
Limitation:
This VIM antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P08670