Cleaved PARP1 Antibody
Code | Size | Price |
---|
NSJ-RQ5288-100ul | 100ul | £535.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: HI-16
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store the Cleaved PARP antibody at -20°C.
Images
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Further Information
Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
Optimal dilution of the Cleaved PARP antibody should be determined by the researcher.
Description:
The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. [Wiki] Cleavage of PARP, by enzymes such as caspases or cathepsins, typically inactivates PARP. While in vitro cleavage by caspase occurs throughout the caspase family, preliminary data suggest that caspase-3 and caspase-7 are responsible for in vivo cleavage. Cleavage occurs at aspartic acid 214 and glycine 215, separating PARP into a 24kDA and 89kDA segment. The smaller moiety includes the zinc finger motif requisite in DNA binding. The 89 kDa fragment includes the auto-modification domain and catalytic domain. [Wiki]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human PARP (24kDa cleavage segment) was used as the immunogen for the Cleaved PARP antibody.
Limitation:
This Cleaved PARP antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P09874