Cleaved PARP1 Antibody

Western blot: 1:500-1:2000

Optimal dilution of the Cleaved PARP antibody should be determined by the researcher.

The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. [Wiki] Cleavage of PARP, by enzymes such as caspases or cathepsins, typically inactivates PARP. While in vitro cleavage by caspase occurs throughout the caspase family, preliminary data suggest that caspase-3 and caspase-7 are responsible for in vivo cleavage. Cleavage occurs at aspartic acid 214 and glycine 215, separating PARP into a 24kDA and 89kDA segment. The smaller moiety includes the zinc finger motif requisite in DNA binding. The 89 kDa fragment includes the auto-modification domain and catalytic domain. [Wiki]

Purified

Antibody in PBS with 0.02% sodium azide and 50% glycerol

A synthetic peptide specific to human PARP (89kDa cleavage segment) was used as the immunogen for the Cleaved PARP antibody.

This Cleaved PARP antibody is available for research use only.

Affinity purified

Human

P09874