Ubiquitin Antibody / K48-Linkage Specific

Western blot: 1:100-1:500, Immunofluorescence/Immunocytochemistry: 1:50-1:200,Immunohistochemistry: 1:50-1:200,Flow cytometry: 1:50

Optimal dilution of the K48-Linkage Specific Ubiquitin antibody should be determined by the researcher.

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. [UniProt]

Purified

Antibody in PBS with 0.02% sodium azide and 50% glycerol

A synthetic peptide specific to Lys-48-linked Ubiquitin was used as the immunogen for the K48-Linkage Specific Ubiquitin antibody.

This K48-Linkage Specific Ubiquitin antibody is available for research use only.

Affinity purified

Human

P0CG47