Bcl-2 Antibody

NSJ Bioreagents
Product Code: NSJ-RQ5323
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5323-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: HF-2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the Bcl-2 antibody at -20°C.

Images

1 / 2
IHC staining of FFPE human spleen tissue with Bcl-2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 2
Western blot testing of human 1) Jurkat and 2) MCF-7 cell lysate with Bcl-2 antibody. Predicted molecular weight ~26 kDa.

IHC staining of FFPE human spleen tissue with Bcl-2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human 1) Jurkat and 2) MCF-7 cell lysate with Bcl-2 antibody. Predicted molecular weight ~26 kDa.

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
Optimal dilution of the Bcl-2 antibody should be determined by the researcher.
Description:
The BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Bcl-2 was used as the immunogen for the Bcl-2 antibody.
Limitation:
This Bcl-2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P10415