GRP78 Antibody / BiP / HSPA5

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ5349
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5349-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: AII-8
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the GRP78 BiP antibody at -20°C.

Images

1 / 2
IHC staining of FFPE human testis tissue with GRP78 BiP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 2
Western blot testing of 1) human HeLa and 2) mouse C6 cell lysate with GRP78 BiP antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
❮❯

IHC staining of FFPE human testis tissue with GRP78 BiP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of 1) human HeLa and 2) mouse C6 cell lysate with GRP78 BiP antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the GRP78 BiP antibody should be determined by the researcher.
Description:
The protein encoded by the HSPA5 gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human GRP78 / BiP / HSPA5 was used as the immunogen for the GRP78 BiP antibody.
Limitation:
This GRP78 BiP antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P11021