GRP78 Antibody / BiP / HSPA5

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NSJ Bioreagents
Product Code: NSJ-RQ5350
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5350-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: BOO-8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the BiP antibody at -20°C.

Images

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IHC staining of FFPE human pancreas tissue with BiP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
2 / 2
Western blot testing of human 1) LNCaP and 2) HepG2 cell lysate with BiP antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
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IHC staining of FFPE human pancreas tissue with BiP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) LNCaP and 2) HepG2 cell lysate with BiP antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the BiP antibody should be determined by the researcher.
Description:
The protein encoded by the HSPA5 gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human HSPA5 / BiP was used as the immunogen for the BiP antibody.
Limitation:
This BiP antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P11021