CD11b Antibody / MAC-1

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NSJ Bioreagents
Product Code: NSJ-RQ5356
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5356-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: GC-9
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the CD11b antibody at -20°C.

Images

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IHC staining of FFPE human spleen tissue with CD11b antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
2 / 2
Western blot testing of mouse RAW264.7 cell lysate with CD11b antibody. Expected molecular weight: 127~170 kDa depending on glycosylation level.
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IHC staining of FFPE human spleen tissue with CD11b antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of mouse RAW264.7 cell lysate with CD11b antibody. Expected molecular weight: 127~170 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the CD11b antibody should be determined by the researcher.
Description:
The ITGAM gene encodes the integrin alpha M chain, also called CD11b and MAC-1. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human CD11b / ITGAM was used as the immunogen for the CD11b antibody.
Limitation:
This CD11b antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P11215